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1 mg vial + 2.5 mL bacteriostatic water: units chart

A 1 mg vial mixed with 2.5 mL of bacteriostatic water holds 400 mcg per mL. This is a dilute mix: each unit holds only 4 mcg, so larger doses take up many units and may not fit a 1 mL syringe. Less water would make them smaller.

Calculate
Vial unit

= 1,000 mcg

mL
Dose unit

= 250 mcg

Syringe type
Syringe size

Units to draw

62.5units

Draw 62.5 units on a U-100 syringe, just above the 62-unit mark.

Syringe filled to 62.5 unitsU-100, 1 mL syringe holding up to 100 units. Draw 62.5 units on a U-100 syringe, just above the 62-unit mark.010203040506070809010062.5
Dose
250 mcg (0.25 mg)
Volume
0.625 mL
Concentration
400 mcg/mL (0.4 mg/mL)
Per unit
4 mcg (0.004 mg)
Full doses in vial
4
Show the math
  1. Convert the vial amount to mcgmg × 1,000 = mcg1 mg × 1,000 = 1,000 mcg
  2. Convert the dose to mcgmg × 1,000 = mcg0.25 mg × 1,000 = 250 mcg
  3. Concentrationvial (mcg) ÷ water (mL) = mcg per mL1,000 mcg ÷ 2.5 mL = 400 mcg/mL (0.4 mg/mL)
  4. Volume to drawdose (mcg) ÷ concentration (mcg/mL) = mL250 mcg ÷ 400 mcg/mL = 0.625 mL
  5. Units to drawmL × units per mL = units0.625 mL × 100 units/mL = 62.5 units
  6. Amount in each unitmcg per mL ÷ units per mL = mcg per unit400 mcg/mL ÷ 100 units/mL = 4 mcg per unit (0.004 mg)
  7. Doses in the vialvial (mcg) ÷ dose (mcg), rounded down1,000 mcg ÷ 250 mcg = 4 full doses

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1 mg peptide with 2.5 mL bac water: units chart

Units to draw on a 1 mL U-100 syringe for common doses, calculated by this site. With this mix, the chart doses from 50 mcg to 400 mcg are easy to read and fit one 1 mL syringe. The 4 largest doses need more than 1 mL.

Dose to units: 1 mg vial with 2.5 mL of water
DoseUnits (U-100)Volume (mL)
50 mcg12.50.125
100 mcg250.25
150 mcg37.50.375
200 mcg500.5
250 mcg62.50.625
300 mcg750.75
400 mcg1001
500 mcg125over 1 mL1.25
600 mcg150over 1 mL1.5
750 mcg187.5over 1 mL1.875
1,000 mcg1 mg250over 1 mL2.5

Key numbers for this mix

Peptide in the vial
1,000 mcg
Concentration
400 mcg/mL
Each unit on a U-100 syringe
4 mcg
Each unit on a U-40 syringe
10 mcg
Smallest easy-to-measure dose (2 units)
8 mcg
Most that fits in a 1 mL syringe
400 mcg

Worked example: 50 mcg

  1. Convert the vial amount to mcg: 1 mg × 1,000 = 1,000 mcg
  2. Concentration: 1,000 mcg ÷ 2.5 mL = 400 mcg/mL (0.4 mg/mL)
  3. Volume to draw: 50 mcg ÷ 400 mcg/mL = 0.125 mL
  4. Units to draw: 0.125 mL × 100 units/mL = 12.5 units
  5. Amount in each unit: 400 mcg/mL ÷ 100 units/mL = 4 mcg per unit (0.004 mg)
  6. Doses in the vial: 1,000 mcg ÷ 50 mcg = 20 full doses

Draw 12.5 units on a U-100 syringe, just above the 12-unit mark. The same 50 mcg dose is 10 units with 2 mL of water and 15 units with 3 mL of water, compared with 12.5 units with 2.5 mL.

Syringe filled to 12.5 unitsA 1 mL U-100 insulin syringe filled to 12.5 units for 50 mcg.010203040506070809010012.5

Frequently asked questions

What is the concentration of a 1 mg vial with 2.5 mL of water?

A 1 mg vial mixed with 2.5 mL of water holds 400 mcg per mL. Each unit on a U-100 syringe holds 4 mcg.

How many units is 50 mcg with a 1 mg vial and 2.5 mL of water?

It is 12.5 units on a U-100 syringe, which is 0.125 mL of the mixed liquid.

What is the smallest dose you can measure well with a 1 mg vial and 2.5 mL of water?

About 8 mcg, which fills 2 units on a U-100 syringe. Smaller amounts are hard to read; a vial mixed with more water spreads them over more units.

What is the largest dose that fits a 1 mL syringe with a 1 mg vial and 2.5 mL of water?

400 mcg, which fills all 100 units of a 1 mL U-100 syringe. Larger doses need more than one draw or a stronger mix.

How many doses does a 1 mg vial with 2.5 mL of water give?

Divide 1,000 mcg by your dose and round down: 20 full doses of 50 mcg, or 10 of 100 mcg.

Nearby vial and water combinations

See all reconstitution charts, or use the peptide calculator for any other vial, water or dose. For the reasoning behind these numbers, read How Much Bacteriostatic Water to Add to a Vial.